All Articles in september, 2025

West Nile virus (WNV), a mosquito-borne pathogen belonging to the Flavivirus genus (family Flaviviridae), is primarily responsible for West Nile fever. In rare cases (< 1% of infections), it can lead to West Nile neuroinvasive disease (WNND), which carries an approximate fatality rate of 10%. This cross-sectional study aimed to assess WNV exposure among residents of Gezira State, Sudan, from 2019 to 2022. A total of 400 participants were included, with 5 mL of blood collected from each—2 mL in an EDTA tube and 3 mL in a plain container. Plasma and serum were separated via centrifugation, and WNV-specific IgG and IgM antibodies were detected using ELISA. The results revealed that 62.25% (249 cases) tested positive for WNV-IgG antibodies, while 37.75% (151 cases) were negative. None of the samples showed detectable WNV-IgM antibodies. Statistical analysis indicated no significant association between WNV-IgG seropositivity and age or gender (p = 0.060 and p = 0.117, respectively). However, significant correlations were observed with marital status and education level (p < 0.05). The study highlights the need for ongoing surveillance of WNV and other vector-borne diseases in the region to identify active West Nile Virus infections. Public health efforts should prioritize mosquito control measures, community education, and awareness campaigns about WNV symptoms and prevention.

Objective: To evaluate the effectiveness of luteal phase support with micronized progesterone alone versus micronized progesterone plus dydrogesterone in patients undergoing in vitro fertilization (IVF) at a human reproduction center located 2,800 meters above sea level.Methods: Retrospective observational study conducted between July 2021 and January 2025 at Hospital Provida (Latacunga, Ecuador). A total of 113 patients with endometrial thickness ≥ 7 mm and serum progesterone < 1 ng/mL before embryo transfer were included. Group 1 received micronized vaginal progesterone 400 mg every 12 h; Group 2 received the same dose plus oral dydrogesterone 10 mg every 12 h. Primary outcomes were biochemical pregnancy (positive β-hCG, defined as ≥ 5 mIU/mL) and clinical pregnancy (fetal heartbeat).Results: Of the total, 40 patients were assigned to Group 1 and 73 to Group 2. Mean age was 38.4 vs 37.7 years, with no significant baseline differences. Biochemical pregnancy rates were 62.5% (monotherapy) and 56.2% (combination), while clinical pregnancy rates were 60.0% and 57.5%, respectively (p > 0.05).Conclusion: Luteal phase support with micronized progesterone alone showed a trend toward higher biochemical and clinical pregnancy rates compared with the combined therapy. These findings support the importance of individualizing luteal phase support strategies in IVF, particularly under physiologically challenging conditions such as high altitude.

Several methods were developed in lab work, including forensic analysis of polymers & organic GSR from 3D printed firearms using Direct Analysis in Real Time Mass Spectrometry (DART-MS) for a wide variety of applications. Since its introduction, DART-MS has been studied for forensic use in areas including drug detection, gunshot residue, printer inks, and even insect identification. We used DART-MS to characterize the polymer evidence left behind by a 3D-printed firearm on cartridge cases, bullets, and the receiving surface. According to James Cizdziel, “There is concern with these 3D printed guns because they have no serial numbers. The fire gun barrels made with Acrylonitrile Butadiene Styrene (ABS), Polylactic Acid, PETG, Chlorinated Polyethylene (CPE) & Nylon were examined by DART-MS. The resulting cartridge cases, bullets & GSR are examined by Direct Analysis of Real Time-Mass Spectrometry”. It successfully detects and identifies the traces of polymer and organic GSR compounds on the bullets, cartridge cases, and in GSR collected from clothing. While detecting polymer in the trace evidence was challenging, DART-MS obtained clear spectra that matched the plastic from the gun from clothing used as a backdrop for test fire. According to the study, DART-MS readily detected three chemicals commonly found in firearm propellants (ethyl centralite, methyl centralite, and diphenylamine) on the bullet and cartridge case, as well as in the solvent wash of the GSR stub. The resulting cartridge cases, bullets, and gunshot residue (GSR) were examined by direct analysis in real time – mass spectrometry (DART-MS). High-resolution mass spectra detected polymer from the gun barrel on bullets and cartridge casings for a 0.38 special Caliber gun and, to a lesser extent, for a 0.22 Caliber 3D-printed gun. This study states that analysis of firearm trace evidence using DART-MS needs more attention & that the technique may be particularly useful for investigating crimes involving 3D-printed guns. I'm writing my review paper on this topic because it's important for addressing challenges related to 3D-printed firearms. These weapons often don't have traditional serial numbers, making them difficult to trace. However, by analyzing polymer residues left on bullets, cartridge cases, or nearby surfaces, investigators can potentially identify the firearm used. The unique chemical makeup of polymers used in 3D printing can help ink the residue back to a specific gun or even a particular 3D printer model. This study states that analysis of firearm trace evidence using DART-MS needs more attention & that the technique may be particularly useful for investigating crimes involving 3D-printed guns.

Influenza is an acute respiratory infection caused by influenza viruses of the Orthomyxoviridae family. Influenza virus cell culture consists of inoculating viral isolates on an MDCK cell layer. The objective of this work is to describe the role of cell culture in influenza virus subtyping during the 2023/2024 influenza epidemic at the Mohammed V Military Teaching Hospital - Rabat.This is a prospective descriptive study carried out at the Virology laboratory of the HMIMV in Rabat, during the 2023/2024 influenza epidemic season (between October and May). It included all samples from patients positive for influenza viruses by the respiratory PCR test. Of the 86 samples positive by PCR, 36 were selected for viral culture.Cell culture allowed the isolation of the virus from the original sample, thus producing a sufficient quantity for further antigenic and genetic characterization, allowing the subtyping of circulating strains.The results showed a predominance of influenza A(H3N2) with 35 cases out of 36 compared to influenza A(H1N1) pdm09 with only 1 case.Cell culture also makes it possible to assess the sensitivity of isolated viral strains to antiviral molecules and to detect the emergence of resistance. Thus, it plays an important role in virological surveillance and in alerting to the event of the appearance of new viruses.

This study comparatively evaluated the analytical performance of the Erba H7100 hematology analyzer against the Siemens Advia 2120i and Beckman Coulter DXH 900, using 243 patient samples. The study assessed the agreement and linear relationship across 14 key hematological parameters in whole blood, employing statistical methods that included mean bias, standard deviation of the difference, Pearson’s correlation, and coefficient of determination. Additionally, reticulocyte counts were analyzed in 27 samples for Erba H7100 vs. Advia 2120i and 53 samples for Erba H7100 vs. DXH 900, revealing exceptional agreement with high Pearson’s r and r-squared values. The performance of the Erba H7100 and DXH 900 in analyzing ascitic, cerebrospinal, and bronchial wash fluids was also evaluated. Notably, the Advia 2120i analyzer exhibited discrepancies in mean corpuscular volume (MCV) and monocyte counts (Mon#). Conversely, the Erba H7100 showed better agreement with the DXH 900 for MCV and Mon# in whole blood. In fluid samples, Erba H7100 and DXH 900 demonstrated a strong correlation with Microscopy in determining Neutrophil % and Lymphocyte % values. Strong linear correlations were observed for most parameters in whole blood, with reticulocyte counts showing near-perfect correlation. This study underscores the importance of rigorous validation and potential platform-specific reference intervals to ensure accurate and reliable hematological testing, emphasizing the need for standardized methodologies in clinical laboratories.

This study comparatively evaluated the analytical performance of the Erba H7100 hematology analyzer against the Siemens Advia 2120i and Beckman Coulter DXH 900, using 243 patient samples. The study assessed the agreement and linear relationship across 14 key hematological parameters in whole blood, employing statistical methods that included mean bias, standard deviation of the difference, Pearson’s correlation, and coefficient of determination. Additionally, reticulocyte counts were analyzed in 27 samples for Erba H7100 vs. Advia 2120i and 53 samples for Erba H7100 vs. DXH 900, revealing exceptional agreement with high Pearson’s r and r-squared values. The performance of the Erba H7100 and DXH 900 in analyzing ascitic, cerebrospinal, and bronchial wash fluids was also evaluated. Notably, the Advia 2120i analyzer exhibited discrepancies in mean corpuscular volume (MCV) and monocyte counts (Mon#). Conversely, the Erba H7100 showed better agreement with the DXH 900 for MCV and Mon# in whole blood. In fluid samples, Erba H7100 and DXH 900 demonstrated a strong correlation with Microscopy in determining Neutrophil % and Lymphocyte % values. Strong linear correlations were observed for most parameters in whole blood, with reticulocyte counts showing near-perfect correlation. This study underscores the importance of rigorous validation and potential platform-specific reference intervals to ensure accurate and reliable hematological testing, emphasizing the need for standardized methodologies in clinical laboratories.

HELLP syndrome is a severe variant of preeclampsia, characterized by hemolysis, elevated liver enzymes, and low platelet count, often resulting in significant maternal and fetal morbidity. One of its most catastrophic but rare complications is hepatic rupture, typically secondary to the development of a subcapsular liver hematoma. This case report describes a patient with hepatic rupture associated with HELLP syndrome, managed at the Leonor Mendes de Barros Maternity Hospital in São Paulo.

The world needs to switch to renewable energy sources in order to fight climate change and make sure energy security. This is a very important chance for developing countries like Ethiopia to use their many natural resources to grow in a good way. Ethiopia has a lot of renewable energy potential, but more than 45% of its people don't have electricity. This is a strange thing about being energy poor. Climate change can cause droughts, which can hurt its grid because it gets 90% of its power from hydropower. Earlier studies looked at Ethiopia's potential for hydropower, geothermal, wind, and solar energy separately. Still, it is important to do a full analysis that includes all sectors within the current policy and investment frameworks. This article goes into great detail about Ethiopia's renewable energy situation. We look at the potential of hydropower, geothermal, wind, and solar energy and come up with policies and investments that will make Ethiopia a regional exporter of renewable energy and provide electricity to everyone by 2030. 

This case report describes a 7-year-old male with gait impairment, frequent falls, and gross motor regression since age 4, despite normal early developmental milestones. Clinical findings included scissoring gait, bilateral lower limb hypotonia, proximal weakness, dystonic right-hand movements, and upgoing plantar reflexes, with normal neuroimaging. Differential diagnoses included cerebral palsy, hereditary spastic paraplegia, dopa-responsive dystonia, and proximal myopathy. Physiotherapy interventions focused on balance, strengthening, gait re-education, and functional independence. After a structured program (45 minutes per session, 5 days/week for 12 weeks), the child demonstrated measurable improvements in GMFM (71.78%), functional independence (FIM: 88), and gait stability, with reduced falls and improved ADL participation. This case highlights diagnostic challenges in pediatric motor disorders and underscores the essential role of physiotherapy in optimizing function despite diagnostic uncertainty.

The aim of this paper is to analyze the role of women in Asturias in October 1934, which has hardly any specific studies. Especially women from the mining working class and politicized young women actively participated in support of men; of course, almost always in functions “appropriate to their sex” (nurses, provisioners of the support of the combatants and caregivers of the children). They were victims of repression by Moors and legionnaires and some fell in combat.

Deaths can result from deliberate self-harm (DSH), accidents, natural causes, homicides, or remain unidentified, causing prolonged distress for the deceased’s family and challenges for authorities. Suicide, a significant public health concern, exemplifies self-destructive behavior often unnoticed or partially noticed. Psychological Autopsy (PA) is highly needed in India due to the significant suicide rate and the complex factors contributing to it. Various nations, including the USA, UK, Canada and Australia, have already recognized psychological autopsy as crucial evidence in court. Although PAs are performed in India, their legal acceptability remains debated. It helps in giving a lesser clouded vision of the victim profile and at times even facilitates the specific definition of the cause of death. Studies reveal that about 90% of those who commit suicide suffer from one or more mental disorders, with depression most common; hence, this finding has been beneficial in identification and treatment of such cases at earliest so as to prevent suicide. Recommendations for the future development of this method include embracing modern communication methods and ‘invisible informants’, cultural intersections, safeguarding of reliability and validity, and the use of feasibility trials. The emphasis remains on collating the raw narratives at the core of these interviews, which make the psychological autopsy such a unique and perceptive tool.

The misuse of topical corticosteroids for skin whitening has grown to be a serious public health issue, notably in South Asia, Africa, and the Middle East. Easy over-the-counter availability, social pressures, and strong cosmetic marketing have all contributed to its widespread use, commonly without medical supervision. This practice has been linked to a number of dermatological problems, including skin atrophy, pigmentary diseases, tinea incognito, and an alarming increase in antifungal resistance to first-line medications like terbinafine. This article examines the incidence of overuse, the underlying pathophysiology, and the dermatological and psychosocial repercussions. Prescription control, improved medical and pharmacy curriculum, digital literacy programs, and appropriate telemedicine utilization are all necessary for effective interventions. Steroid abuse is more than just a cosmetic issue; it is a developing dermatological and antimicrobial resistance hazard that requires immediate, concerted action.

Background: Enhanced Recovery After Surgery (ERAS) protocols are comprehensive, interdisciplinary frameworks aimed at minimizing surgical stress, standardizing perioperative care, and expediting recovery. Perianesthesia nurses, who operate in preoperative assessment units and post-anesthesia care units (PACU), play a vital role in ensuring the integrity of ERAS implementation. Objectives: To compile and analyze current evidence regarding the impact of perianesthesia nursing care on surgical outcomes pre- and post-anesthesia, and to compare outcomes of ERAS with traditional care practices. Methods: A narrative review of ERAS guidelines along with recent meta-analyses and clinical trials (2016–2025) focusing on nursing-sensitive processes (such as patient education, anxiety management, adherence to PONV prophylaxis, multimodal pain management, early mobilization, and readiness for discharge) as well as outcomes (including length of hospital stay, complications, readmission rates, and time to PACU discharge). Results: Meta-analyses across different specialties indicate that the adoption of ERAS correlates with reduced hospital stays and a decrease in complications without an increase in readmission rates or mortality. Processes led by perianesthesia nurses—including the education of patients and setting their expectations, adherence to carbohydrate loading and fasting guidelines, maintenance of normothermia, PONV prophylaxis based on risk assessment, opioid-sparing pain management, protocols for the removal of urinary catheters/lines, and encouragement of early oral intake and ambulation—contribute to these positive outcomes. Research also reveals that ERAS programs facilitate quicker fulfillment of discharge criteria in the PACU. Conclusion: Perianesthesia nurses implement ERAS protocols at the bedside, effectively converting these guidelines into dependable practices that enhance recovery milestones and diminish complications. Ongoing benefits are contingent upon systematic documentation, effective interprofessional communication, and continuous performance audit and feedback.

Chimerism is a biological condition in which a single individual harbors two or more genetically distinct cell populations originating from different zygotes. This phenomenon may occur naturally due to errors during fertilization or early embryonic development, or it may arise artificially following medical interventions such as hematopoietic stem cell transplantation (HSCT) or in vitro fertilization (IVF). Chimerism is broadly categorized as natural or artificial, and its presence presents significant challenges in both clinical and forensic contexts. In transplant recipients, the coexistence of donor- and host-derived cells can generate misleading genetic test results and complicate post-transplant monitoring. In forensic investigations, individuals with mixed DNA profiles may be difficult to identify accurately, as standard genetic fingerprinting technologies can yield inconclusive or erroneous findings. These complications underscore the necessity of reliable detection methods capable of identifying and differentiating chimeric cell populations. This review consolidates current knowledge regarding the classification of chimerism and the available diagnostic techniques, emphasizing that improved understanding of this condition is essential for enhancing diagnostic precision, optimizing forensic identification, and minimizing the risk of misinterpretation that may adversely affect medical decisions and legal determinations.

The resistance to anthelmintics in poultry farming and the challenges with the restricted use of drugs in organic farms make the use of biological controllers an innovative bridge to verminosis control. This paper aims to evaluate the efficacy of the larvicidal fungus Duddingtonia flagrans and the ovicidal fungus Pochonia chlamydosporia in Hy-line Brown (Gallus Gallus Domesticus) layer farms. Both fungi were combined in the core of the feed administered to the birds of the treated groups. 28,000 birds were used and divided into three treated groups (TG) in which the feed containing the fungus was administered. The poultry house itself manufactures the birds’ feed; the formula was included in the nucleus at a dose of 100 grams per ton of feed for 7 months. The concentration was 105 chlamydospores of P. chlamydosporia and D. flagrans per gram of the formulation. The control group (CG) received regular food from the farm. The birds were separated into four sheds with 7,000 birds in each. The egg per gram of feces (EPG) testing was performed using fresh fecal samples collected from the sheds over six months. Weather data was collected during the experiment. There was a reduction in the EPG count into three treated groups. The most prevalent helminth was the genus Ascaris. The formulation tested shows little efficacy in this dosage. Key points:•    The use of the fungus P. chlamydosporia and D. flagrans in organic farms;•    Use of biocontrol agents without chemicals in poultry;

One of the most important and critical red blood cell disorders is dysfunction and deformation of the membrane structure, which affects the metabolic and biological red blood cell functions. On the other hand, the basic causes of these problems are the genetic mutations in the production of proteins that correlate to the structure and receptors of cells. The diagnosis methods and techniques are the other essential points that focus most scientists on. In this systematic review, the article pointed to the key title, which is the diagnosis of novel genes with different techniques and methods. The result of articles studies that were published in the last decades underlined the types of techniques such as Whole-Exome Sequencing, Quantitative Real-Time PCR, Targeted Next-Generation Sequencing, and Sulphate-Polyacrylamide Gel, which investigated the different membraned gene mutations that are novel and correlated to the genes that make the structures and functions of red blood cells such as hereditary spherocytosis, hereditary elliptocytosis, hereditary pyro poikilocytosis. In addition, the hereditary membrane disorders correlated to others, such as hereditary spherocytosis, have a relationship with vitamin B12, immunodeficiency.

The 2025 AHA/ACC/AANP/AAPA/ABC/ACCP/ACPM/AGS/AMA/ASPC/NMA/PCNA/SGIM guideline for the prevention, detection, evaluation, and management of arterial hypertension introduces important updates compared with the 2017 version. Given the global impact of hypertension as a major cause of cardiovascular morbidity and mortality, and the coexistence of multiple international and national recommendations, the Mexican Expert Group on Arterial Hypertension (GREHTA) conducted a structured review and comparative analysis. We contrasted the 2025 AHA/ACC recommendations with those of the European Society of Hypertension (ESH 2023), the European Society of Cardiology (ESC 2024), the Latin American Society of Hypertension (LASH 2024), and the National Medical Care Protocol of Mexico (PRONAM 2025). Our goal is to provide clinicians with a clear, evidence-based synthesis to guide practical decision-making. Key topics include definitions and thresholds, diagnostic methods, cardiovascular risk stratification, therapeutic strategies, special populations, and resistant hypertension. GREHTA emphasizes a pragmatic, regionally adapted approach, highlighting early combination therapy, risk factor integration, and systematic follow-up.

This study proposes a new type of flange-widened bone-type semi-rigid frame node, and takes a 4-story frame as an example, uses the finite element software Abaqus  to analyze its seismic performance  under different seismic waves, and compares it with the traditional rigid connection frame. The results show that the stiffness of the new node frame is slightly lower than that of the tradition rigid node frame, which reduces the horizontal displacement of each layer under the action of earthquake, optimizes the deformation capacity, and enhances the force performance and ductility, the maximun value of the base shear force is lower than that of the traditional rigid frame, which can effectively disperse and dissipate the seismic energy andimprove the overall stabitity of the structure; the elastic-plastic interlayer displacement angle is less than 1/50 under rare earthquakes, which meets the requirements of the specification and can achieve the goal of “no collapse in a large earthquake”. In addition, the node can effectively improve the safety and reliability  of the structure in actual engineering.

The article evaluates the features of the course of chronic HBV infection in children with the background of anemia of inflammation. A brief description of the clinical and laboratory manifestations of the disease is given, depending on the course of anemia and inflammation. The data on hepcidin dynamics are analyzed. The stage–by-stage formation of iron metabolism disorders was revealed, in the form of a true deficiency with a breakdown of ferrokinetic markers – an increase in hepcidin and soluble transferin receptors with the background reduced ferritin values characteristic of iron deficiency anemia in the initial stages of the disease and, redistributive iron deficiency - decrease in hepcidin and soluble transferrin receptors with the background increased ferritin values characteristic of iron overload the body is in the late stages of the disease.

The research was conducted in southern Serbia, at an altitude of 600 meters, in a beech stand, on a dwarf albino specimen furher usually called Absiente pigmente fagus or just Absiente pigmente. Soil sampling and analysis were carried out at depths of 0-10 cm, 11-20 cm, and 21-30 cm. Laboratory tests included determination of the textural composition of the soil, total humus content, total nitrogen, total phosphorus and potassium, the sum of absorbed base cations, determination of hydrolytic acidity, and both active and substitutional acidity. The soil was found to have a sandy textural composition, with a strong to very strong acidic reaction and a low degree of saturation with base cations. It was further determined to be highly humic, well-supplied with total nitrogen and plant-available potassium, but poorly supplied with phosphorus. Chemical composition analyses of the leaves were simultaneously conducted on both pigmented and non-pigmented leaves. The composition of 15 macro and microelements was examined. The results showed that their concentration in albino leaves was 30% - 50% higher, except in the case of Se. The presence of heavy metals such as cadmium, chromium, and nickel was significantly higher in the non-pigmented beech tissue compared to the green tissue (38.56% for Cd, 22.97% for Cr, and 44.89% for Ni). The research was conducted for the purpose of heavy metal extraction from the soil and green restoration, and it shall continue to this end.

Dental implantology represents a cornerstone of modern restorative dentistry, providing long-term functional and esthetic rehabilitation of missing teeth. The process of osseointegration—first introduced by Brånemark—refers to the direct structural and functional connection between living bone and the surface of a load-bearing implant [Brånemark, 1985].

Introduction: Necrotizing Fasciitis (NF) is a rapidly progressing and life-threatening soft tissue infection, exceedingly rare but often fatal in neonates. This case series highlights the rarity, fulminant nature, and poor prognosis of neonatal NF by presenting four cases.Case presentation: Four neonates, aged 12-16 days, presented with rapidly spreading, tender, erythematous, and indurated skin lesions on their backs, initially resembling burns. Systemic symptoms like fever, lethargy, and poor feeding were common. Despite empirical antibiotics, the lesions progressed to necrosis, often with bullae formation. Microbiological cultures revealed polymicrobial growth in three cases (E. coli, Pseudomonas sp., Klebsiella, and MRSA) and monomicrobial growth of MRSA in one case, frequently exhibiting antibiotic resistance. Surgical debridement was performed in three cases. Despite aggressive management, two neonates succumbed to sepsis and multi-organ dysfunction. The other two neonates recovered after prolonged antibiotic therapy and wound care.Discussion: These cases underscore the diagnostic challenges and rapid progression of NF in neonates. The consistent presentation after 10 days of birth, rapid lesion spread mimicking burns, and predilection for the back were notable features. Polymicrobial infection was frequent. Early recognition, aggressive broad-spectrum antibiotics, and timely surgical debridement are crucial for improving the poor prognosis associated with this condition.