Evolving Paradigms in Strep Throat: From Epidemiology to Advanced Therapeutics - A Comprehensive Overview
Published on: 29th December, 2022
Strep throat, a common affliction known for its hallmark symptom of a severe and sudden sore throat, is caused by the bacterium Streptococcus pyogenes, classified under Group-A Streptococcus (GAS) [1]. This condition not only impacts millions globally but also carries the risk of severe complications if left untreated [2]. Understanding strep throat goes beyond recognizing its symptoms; it entails an appreciation of its transmission dynamics, potential complications, and the evolving landscape of treatment and prevention strategies [3].
Time Electron Theory
Published on: 5th April, 2024
What is time? Is it a physical quantity, illusion, or dimension? Defining time is challenging and fascinating. We often consider time as a dimension to help us understand the concept of space-time. Time undeniably exists, but we can only sense its presence through its effects. For instance, if we take two apples, one bought months ago and the other just a day ago, we can tell that one apple was bought a long time ago because it had rotted. We express time as the effect caused by it. If there were no effects of time on this universe, then the concept of time would not exist [1,2].
Statistical Mathematical Analysis of COVID-19 at World Level
Published on: 5th April, 2024
Worldwide, statistical data of people infected by COVID-19 has been taken until March 29, 2023, which, when correlated, showed a predictive logistic model. The purpose was to determine the predictive model, which was acceptable, in such a way that the proportionality constant and the correlation and determination coefficients are of great importance to estimating epidemiological and pandemic data; coinciding with what was reported by other authors. Bearing in mind that a mathematical model is a mathematical description through a function or equation of a phenomenon in the real world; whose purpose is to understand infections and make predictions for the future. The stages were: to model the number of people infected as a function of time, formulate, and choose the logistic model, determine the model and obtain mathematical conclusions, and make predictions (estimates) about the number of people infected by COVID-19 worldwide. The logistic model was derived to predict the speed of people infected by COVID-19 and the critical time (tc = 733 days) for which the speed was maximum (1694,7209 infected/day). The Pearson correlation coefficient for the time elapsed (t) and the number of people infected (N) worldwide, based on 32 cases, was r = -0.88; the relationship between time and those infected is real, there is a “very strong correlation” between the time elapsed (t) and the number of people infected (N) and 77.03% of the variance in N is explained by t.
Effect of Methyl Jasmonate on the Expression of Transcription Factors in Wild Jujube Seedlings under Salt Stress
Published on: 8th April, 2024
Methyl Jasmonate (MeJA) can be used as a signal molecule to regulate the expression of resistance genes in the resistance to abiotic stress, thus improving the salt tolerance of wild jujube. Among the resistance genes combined with methyl jasmonate, transcription factors play an important role in response to salt stress. However, the interaction of transcription factors in different tissues under salt stress and the regulation of transcription factors by MeJA remain unclear. In this study, the effects of MeJA on transcription factor expression in wild jujube under salt stress were investigated, and the differences in transcription factor expression among different tissues were compared. It was found that MeJA could increase the type and quantity of transcription factors responding to salt stress. The types of transcription factors responding to salt stress were roughly the same among different tissues, but the quantity and expression of the transcription factors were significantly different. The results of transcription factor co-expression analysis showed that transcription factors play synergistic roles in the face of abiotic stress, which can provide preferable genes for subsequent transgenic work.
COVID-19 Pneumonia in Pregnancy: A Retrospective Study on Maternal and Neonatal Outcomes
Published on: 8th April, 2024
Objective: To identify risk factors among pregnant with COVID-19 for adverse outcomes related to disease severity, maternal mortality, and morbidity.Materials and methods: In this retrospective study, 45 pregnant patients with COVID-19 pneumonia were confirmed by RT-PCR. The inclusion criteria were pregnant patients diagnosed with COVID-19 confirmed by RT-PCR and hospitalized in the gynecology-obstetrics and intensive care unit. Exclusion criteria were non-pregnant patients and pneumonia cases with unconfirmed COVID-19 causes. The study used SPSS software to analyze the data. Results: Our study recorded 45 cases of SARS-CoV-2 infection in pregnant women over 2.5 years. The age group most affected was 20-35 years, with 75% of cases. 57% of patients had no known comorbidities. 88.8% of patients were symptomatic at diagnosis. Almost 30% of patients required admission to the ICU, with 60% requiring oxygen supplementation. The study recorded 36 live births (80%), of which 26 cases (72.2%) required no further care and had a favorable outcome.Conclusion: Pregnant women with medical conditions are at higher risk of severe COVID-19, which can cause respiratory distress syndrome and impact delivery and neonatal outcomes. Preventive measures are important.
Proportion of Risk of Stroke among Male Patients Attending Outpatient Department of a Tertiary Care Hospital, Ernakulam District, Kerala
Published on: 9th April, 2024
Aim: A descriptive-analytical study was done to assess the proportion of the risk of stroke among male patients attending the outpatient department of a tertiary care hospital, in Ernakulam District, Kerala. Objectives: The objectives of the study were to assess the proportion of the risk of stroke among male patients attending outpatient departments and to determine the association of stroke risk level with socio-personal variables. Methods: The research approach of the study is a quantitative approach. The research design selected for the study is a descriptive-analytical design. The convenience sampling method was used for sample selection. The study was done in MOSC Medical College Hospital, Kolenchery among 288 male patients who met the inclusion criteria. Data collection was done using socio-personal proforma and stroke risk scorecards. Results: Collected data were analysed by using descriptive and inferential statistics presented in tables and pie diagrams. The study findings revealed that out of 288 subjects, 43.4% have low stroke risk, 13.9% are in caution and 42.7% have high stroke risk. The findings also revealed that there is a significant association of stroke risk level with age, education, occupation, BMI and place of residence. Statement of the problem: Proportion of risk of stroke among male patients attending outpatient department of a tertiary care hospital, Ernakulam district, Kerala. Proportion of risk of stroke among male patients attending outpatient department of a tertiary care hospital, Ernakulam district, Kerala.
Sites and Zones of Maximum Reactivity of the most Stable Structure of the Receptor-binding Domain of Wild-type SARS-CoV-2 Spike Protein: A Quantum Density Functional Theory Study
Published on: 12th April, 2024
Today, it is well known that Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has four types of proteins within its structure, between them the spike protein (S). The infection mechanism is carried out by the entry of the virus into the human host cell through the S protein, which strongly interacts with the human cell receptor angiotensin-converting enzyme 2 (ACE2). In this work, we propose an atomic model of the Receptor Binding Domain (RBD) of the S spike protein of the wild-type SARS-CoV-2 virus. The molecular structure of the model was composed of 50 amino acids that were chemically bonded, starting with Leucine and ending with one amino acid Tyrosine. The novelty of our work lies in the importance of knowing the sites and zones of maximum reactivity of the RBD from the fundamental levels of quantum mechanics considering the atomic structure of matter. For this, the local and global reactivity indices of the RBD were calculated, such as frontier orbitals, Highest Occupied Molecular Orbital (HOMO) and Lowest Unoccupied Molecular Orbital (LUMO), Fukui indices, chemical potential, chemical hardness, electrophilicity index; with this, it will be possible to know what type of molecules are more likely to interact with the RBD structure, and in this way, new knowledge will be generated at the quantum, atomic and molecular level to inhibit the virulent effects of wild-type SARS-CoV-2. Finally, in order to identify the functional groups within the most stable structure and thereby verify the future reactions that can be carried out between the RBD structure and biomolecules, the Infrared (IR) absorption spectrum was calculated. For this work, we used Material Studio v6.0 which uses the density functional theory (DFT) implemented in its DMol3 computational code. The IR spectrum was obtained using the Spartan ‘94 computer code. One novelty would be that we found nine amino acids more that could make the RBD and ACE2 binding further the already known. Thus, the Mulliken charge distribution indicates that the highest concentrations of positive and negative charge are found in the zones 477S, 478T, 484E, and 501N amino acids letting ionic or Van der Waals possible interactions with other structures.
New Fungi Associated with Blackberry Root Rot (Rubus spp.), in Michoacán, Mexico
Published on: 15th April, 2024
Los Reyes, Michoacán, Mexico, is one of the main blackberry-producing places in the world, however, the disease located at the root level has caused important economic losses. Currently has been reported that the fungus Fusarium spp., is the main causal agent but actions to control it have failed. The objective of this work was to identify the possible presence of unreported pathogenic fungi in the root system of the blackberry and identify them molecularly. It was sampled in a commercial open-air orchard from Los Reyes, pieces of roots were taken from symptomatic plants with wilting and decay. The fungi were isolated in the laboratory, identified with taxonomic keys, extraction was performed, and the sequences obtained were compared with those reported in the NCBI gene bank. Among the results obtained were Kalmusia italica, Epicoccum nigrum, Microsphaeropsis arundinis, Achizophyllum commune, and, as expected, some species of Fusarium spp.
Are Biofungicides a Means of Plant Protection for the Future?
Published on: 15th April, 2024
Biofungicides are prepared based on living micro/organisms or on matters prepared from them. They are based on the antagonism of fungal pathogens and their antagonists. Their effect depends on weather conditions (temperature and moisture) in comparison with chemical fungicides which are effective in all conditions but they let the residues in plants, animals, and men. The future of agriculture will be pure food without chemicals.
Hypo-glycaemia and Associated Factors among Type 1 Diabetes Mellitus Patients Attending the Outpatient Clinics of Debre-tabor Compressive and Specialized Hospital, South Gondar Zone, North West Ethiopia: A Cross-sectional Study
Published on: 15th April, 2024
Introduction: Hypoglycemia affects patient safety and glycemic control during insulin treatment of both type 1 (T1DM) and type 2 diabetes mellitus (T2DM). It is still a major clinical problem in the treatment of type 1 diabetes that impairs metabolic control. This study aimed to assess hypoglycemia and associated factors among type 1 diabetes mellitus patients attending the outpatient clinics of Debra–Tabor Comprehensive and Specialized Hospital, in 2021.Method: institutional-based cross-sectional study design was conducted among 204 selected type I DM, from June 1/2021 to August 30/2021. The study participants were selected by systematic sampling method from monthly follow-up lists and lists of registration. Data were cleaned and entered into Epi Data and then exported into SPSS version 20 for analysis. Data were presented with texts, graphs, diagrams, and tables but an analytic form of findings will be presented by text form of the odds ratio. Bivariable and multivariable binary logistic regression models were fitted to identify factors associated with hypoglycemia p – value < 0.05 was considered statistically significant and reported as a 95% confidence interval (CI).Results: A total of 204 participants participated with a response rate of 100%, and prevalence of self-reported hypoglycemia was 88.7% (95%, CI: 83.8-92.6) and the prevalence of hypoglycemia was significantly associated with occupation [AOR: 4.01 (95% CI:1.86-9.35)], higher educational status [AOR: 2.13 (95% CI:1.92-13.15)], diabetic duration < 1 [AOR: 3.80 (95% CI:1.32-9.28)], poor knowledge [AOR: 4.91 (95% CI:1.09-11.06)] and favorable attitude [AOR: 5.86 (1.10-6.07)]. The current study revealed that the knowledge, attitude, and practice of respondents were 81.9% (95%, CI, 76-86.8), 73% (95% CI, 66.7-78.9), and 91.2% (95% CI, 86.8-94.6) respectively.Conclusion and recommendation: Hypoglycemia was highly prevalent among diabetic patients treated at Debra-Tabor Comprehensive and Specialized Referral Hospital. To reduce the higher prevalence of hypoglycemia the health professionals working in the hospital had better provide appropriate patient advice about, BGL monitoring, medication adherence, self-injection as well as oral hypoglycemic agent administrations.
Chronic Ethanol Decreases the Left Ventricular Cardiac Performance
Published on: 15th April, 2024
Introduction: Spaced fiber bundles, less evident cell striations, deposition of collagen fiber bundles, and increased presence of fibroblasts in the cardiac tissue of rats that voluntarily and chronically ingested ethanol were observed by our research group. In addition, we observed increased proliferation and cell death of cardiomyocytes. To find out whether these changes lead to impaired heart functionality, some clinical tests were performed. Aim: to investigate whether chronic ethanol decreases the left ventricular performance assessed by exercise testing, electrocardiogram, and echocardiogram of male and female UChB strain rats. Material and methods: ten adult males and ten adult females, Wistar strain rats, named UChB (University of Chile), spontaneous high ethanol drinkers (consumption greater than 2 g ethanol / Kg body weight/day), and ten male UChB rats and ten adults female UChB rats, not exposed to ethanol, UChBC rats (controls) were used. Cardiac performance was evaluated by the stress test, electrocardiogram, and echocardiogram. Results: Exposed females showed ventricular morphological changes in the heart. The exposed females and males had the highest heart relative weight with females being larger than males. The exposed females showed altered electrocardiogram and echocardiogram. Conclusion: Chronic ethanol decreases the left ventricular cardiac performance in rats. Females are more sensitive to changes in cardiac electrical conduction.
Long monitoring of Birds of Elssuki Area. Sinnar State, Sudan
Published on: 16th April, 2024
This study aims to make a database of the birds in the area of Elssuki, Sinnar state - Sudan. The period of study spanning from 2008 to 2023 with a mix of methods used to identify bird species in many sites in the locality, these methods include road count, line transects, and direct count besides registering every strange, rare, or unusual single species seen in the area. All these methods are used by different researchers and applied in such studies in Sudan.The study revealed that the area is one of the important areas enriched of birds in Sinnar state of 19 orders 53 families. The total number of species is 129 species. It included all birds; water birds, tree birds, diving birds, dabbling birds, swimming birds, small waders, and passerines which the most. The study concluded that there is a need for comprehensive and regular studies and short and long-term monitoring to identify, classify, and establish a database for Sudan Birds Atlas.
Morning and Evening Exercise with or without High-Pressure Intervals have Different Effects on Controlling Blood Sugar and Glucose Fluctuations in People with Type 1 and Type 2 Diabetes
Published on: 29th December, 2023
Summary: Exercise is recommended for the treatment and prevention of type 2 diabetes. Also, to control and reduce glucose fluctuations in people with type 1 diabetes. However, the most appropriate time and the most effective intensity of exercise is still unknown, and various studies provide different results and different recommendations, and none of the studies provide a comprehensive and practical result. We conducted our studies to examine the results and determine the effect of time and intensity of exercise on blood sugar control and glucose fluctuations during the day. Methods: search in PubMed and Google Scholar with keywords morning, evening, type 1 and 2 diabetes, exercise, interval, periodic, aerobic, and glucose and blood sugar fluctuations were performed. A total of 31 articles were reviewed and finally, 10 articles that were most related to each other or had complementary information were selected. Conclusion: HIIT exercises are useful for type 2 diabetes, but they are recommended for type 1 diabetes with less pressure. Morning exercise increases glucose and evening exercise is applicable for type 1 and 2 diabetes.
Exploring the Barriers to Clinic-based Screening for Sexually Transmitted Infections (STIs) among Men in Ghana: A Qualitative Study
Published on: 16th April, 2024
The burden of sexually transmitted infections (STIs) continues to increase with over one million curable STIs occurring daily worldwide. Sex disparity in the rates of testing for STIs can compromise the efforts to reduce the incidence of STIs. The study aimed to explore the barriers to facility-based screening for STIs among men in Ghana. A qualitative exploratory design was employed in this study. Using a semi-structured interview guide, individual in-depth interviews were conducted among purposively sampled men from November 2019 to January 2020. Data saturation was achieved at the ninth participant. Audio-recorded interviews were transcribed verbatim and analysed thematically through Braun and Clarke’s approach. Four themes emerged from the analyses: (1) lack of privacy from healthcare providers, (2) mistrust of healthcare providers, (3) the burden of handling thoughts of positive status, and (4) fear of stigmatisation. These barriers deterred men from seeking clinic-based screening for STIs. The barriers were multi-factorial and a major hindrance to ensuring that people are aware of their STI status through screening and diagnosis. It is imperative to consider these barriers when designing STI screening interventions and policies to help promote facility-based screening for STIs among men in Ghana.
Primary Diffuse Leptomeningeal Melanocytosis: A Rare and Challenging Diagnosis
Published on: 17th April, 2024
Primary melanocytic neoplasms of the central nervous system are rare entities and can present in different clinical forms with mild and non-specific symptoms (such as headache and tinnitus) to severe and limiting symptoms (focal deficits and intracranial hypertension), mimicking the most diverse pathologies. In addition to the peculiar changes in imaging tests, diagnosis is always a challenge given the multitude of possible differential diagnoses, including aseptic meningitis. Given this, we bring here the case of a 59-year-old patient who attended care due to headache and vertigo followed by involvement of the cranial nerves and spinal cord, corroborated by physical examination and imaging study suggesting diffuse involvement of the meninges, which was subsequently confirmed by anatomopathological examination as a primary melanocytic neoplasm of the central nervous system but ended up dying due to complications resulting from late diagnosis. The objective of this work is to raise awareness about the possibility of this pathology as a differential diagnosis in these cases where there are often frustrating clinical manifestations but with changes in imaging tests, to enable an early diagnosis and consequently the possibility of a better therapeutic result, in addition to a brief review of the propaedeutic findings of this pathology.
The Potential Use of Dimethyltryptamine against Ischemia-reperfusion Injury of the Brain
Published on: 19th April, 2024
Ischemia-Reperfusion Injury (IRI) is the outcome of two intertwined pathological processes resulting from the shortage of blood flow to tissues and the subsequent restoration of circulation to a previously ischemic area. IRI (sometimes just one side of the dyad) remains one of the most challenging problems in several branches of emergency medicine. Mitochondrial and endoplasmic reticulum dysfunction is a crucial pathological factor involved in the development of IRI. The sigma-1 receptor (Sig1-R) is an intracellular chaperone molecule located between the mitochondria and endoplasmic reticulum with an apparent physiological role in regulating signaling between these cell organelles and serves as a safety mechanism against cellular stress. Therefore, amelioration of IRI is reasonably expected by the activation of the Sig1-R chaperone. Indeed, under cellular stress, Sig1-R agonists improve mitochondrial respiration and optimize endoplasmic reticulum function by sustaining high-energy phosphate synthesis. The discovery that N, N-dimethyltryptamine (DMT) is an endogenous agonist of the Sig1-R may shed light on yet undiscovered physiological mechanisms and therapeutic potentials of this controversial hallucinogenic compound. In this article, the authors briefly overview the function of Sig1-R in cellular bioenergetics with a focus on the processes involved in IRI and summarize the results of their in vitro and in vivo DMT studies aiming at mitigating IRI. The authors conclude that the effect of DMT may involve a universal role in cellular protective mechanisms suggesting therapeutic potentials against different components and types of IRIs emerging in local and generalized brain ischemia after stroke or cardiac arrest.
Unlocking the Transformative Power of Synthetic Biology
Published on: 18th April, 2024
Artificial Intelligence (AI) combined with Synthetic Biology has the potential to change the way we approach medicine, agriculture, and manufacturing. AI automates tasks, optimizes experimental designs, and predicts biological behaviours, resulting in more efficient design and engineering of biological systems. However, there are challenges such as data limitations, interpretability issues, and ethical considerations like biosafety and biosecurity concerns that need to be addressed. AI can be used to analyze vast amounts of data and identify patterns. This has led to successful applications of AI in high-throughput screening and biomanufacturing, which can drive innovation and address critical challenges. AI-powered closed-loop systems for real-time monitoring and control of biological processes also show promise in providing real-time feedback and optimizing systems on the fly. Despite these advancements, it's important to consider ethical implications to ensure the responsible development and application of AI in synthetic biology. Proper consideration of challenges and ethical considerations can help leverage the power of AI to drive innovation and tackle pressing societal challenges. Overall, the potential of AI in synthetic biology is significant. By addressing challenges and ethical considerations, we can use them effectively to solve pressing problems.
Macitentan in Adults with Sickle Cell Disease and Pulmonary Hypertension: A Proof-of-Concept Study
Published on: 22nd April, 2024
Pulmonary hypertension (PH) in sickle cell disease (SCD) is associated with a mortality rate of 37%. There is an upregulation of adhesion molecules which leads to the expression of endothelin-1, a potent vasoconstrictor. A prospective, descriptive study was done to determine the safety and efficacy of macitentan in patients with SCD and PH. Continuous variables were reported as mean ± SEM or percentage where appropriate. We screened 13 patients and recruited five. All five patients were adults. Data were analyzed as appropriate by student t - test. Statistical significance was assumed at p < 0.05. Baseline pulmonary hemodynamics obtained by right heart catheterization and systemic hemodynamics were (± SEM): mean pulmonary artery pressure (MPAP) 32 ± 8 mmHg, right atrial pressure (RAP) 9 ± 4 mmHg, pulmonary vascular resistance (PVR) 257 dynes-sec/cm5 and CI 3·7 ± 0.39 l/m2. Of all parameters, only PVR and 6-min walk distance changed significantly. For the group, MPAP decreased by 15.6%, PVR by 22.5% and RAP by 25.5%. The 6-minute walk distance increased over sixteen weeks except in Patient 4 who had a 3% decrease. The mean walk distance increased in the total distance, from 464 ± 158 meters to 477 ± 190 meters (p .123). In four patients, the adverse events were mild to moderate and did not lead to study drug discontinuation. Significant improvement in pulmonary hemodynamics and exercise capacity in patients with SCD-related pulmonary arterial hypertension. We found that macitentan was safe and well tolerated.
Breast Cancer in Female
Published on: 22nd April, 2024
Anxiety is also a very common disorder, both in patients and their family members. Anxiety and stress can compromise the quality of life of cancer patients and their families. Feelings of anxiety and anguish can occur at various times of the disease path: during screening, waiting for test results, at diagnosis, during treatment or at the next stage due to concern about relapses. Anxiety and distress can affect the patient’s ability to cope with diagnosis or treatment, frequently causing reduced adherence to follow-up visits and examinations, indirectly increasing the risk of failure to detect a relapse, or a delay in treatment; and anxiety can increase the perception of pain, affect sleep, and accentuate nausea due to adjuvant therapies. Failure to identify and treat anxiety and depression in the context of cancer increases the risk of poor quality of life and potentially results in increased disease-related morbidity and mortality [1]. From all this we deduce the need and importance of dedicated psychological and psychiatric support for these patients within the Breast Unit. The fact that the psycho-oncologist who is dedicated to the care of patients with breast cancer must be an integrated figure in the multidisciplinary team of the Senological Center and not an external consultant is enshrined in the same European Directives that concern the legislation concerning the requirements that a Breast Unit must have in order to be considered a Full Breast Unit (Wilson AMR, et al. 2013).One of the most complex situations you find yourself dealing with is communication with the patient. This communication is particularly complex in two fragile subpopulations that are represented by women. [Menditto L. T (Tirannie) Cancer of the Breast. Am J Psychol & Brain Stud, 2023; 1(1):26-30].
Designing a Community Health Worker (CHW) Certificate Training that Centers Marginalized Youth’s Health and Wellness
Published on: 23rd April, 2024
The genesis of the youth Community Health Worker (CHW) and Citizen/Community Science (CS) training is rooted in interdisciplinary research and the lived experiences of families in Chicago and Urbana-Champaign. Dr. Mendenhall and colleagues’ (Drs. Robinson, Roberts and Rodriquez-Zas) South Chicago’s Black Mothers’ Resiliency Project provide dinsights into Black women’s experiences with genomics, trauma, and community science. Black women’s testimonies highlighted the health impacts of living in neighborhoods with high levels of gun violence and the resilience strategies they employ. This led to the development of a youth-centered CHW and CS training program, aimed at addressing health disparities. The program trained high school students and young adults (up to age 24) as CHWs and CSs, focusing on the objectives of (1) embedding culturally competent health workers in marginalized communities; (2) amplifying community voices; (3) collecting, analyzing, and quantifying observational data to inform policy decision-making; (4) amplifying community voices in healthcare discussions and (5) creating community health care worker training certification for youth. Over three years, the program trained over 50 participants, incorporating elements of wellness, art, and entrepreneurship.Based on ethnographic notes participants reported increases in wellness such as reduced anxiety and increased empowerment. The program’s long-term goals include creating employment opportunities for graduates and contributing to improved community health outcomes. This initiative represents a step towards addressing racial trauma and promoting community healing through youth-led innovation and empowerment.
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