Yosberto Cárdenas-Moreno*, Luis Ariel Espinosa, Julio Cesar Vieyto, Michael González-Durruthy, Alberto del Monte-Martinez, Gilda Guerra-Rivera and Maria Isabel Sánchez López
Laccase catalyzes oxidation of lignin and aromatic compound with similar structure to this one. Their low substrate specificity results on degradation of similar phenolic compounds. In this context, Molecular Docking was performed with different ligands suggesting potential bio-degradation. Binding active-sites prediction of fungal laccase (access number uniprotkb: A0A166P2X0), from Ganoderma weberianum was performed using machine learning algorithm based on Deep Convolutional Neural Networks (DeepSite-CNNs chemoinformatic tool). Herein, ligands like 2,4 - dichlorophenol, benzidine, sulfisoxazole, trimethoprim and tetracycline were analyzed and two additional reference controls which were 2,2 – azinobis 3 – ethylbenzothiazoline – 6 - sulfonic acid (ABTS) and 2,6 - dimetoxyphenol (2,6 DMP) were used in comparison with the other former mentioned ligands based on high laccase affinity. The five ligands were carried out because their potential biotechnological interest: the antibiotics sulfisoxazole, trimethoprim and tetracycline, and xenobiotics 2,4 - dichlorophenol and benzidine. Molecular docking experiments returned Gibbs free energy of binding (FEB or affinity) for laccase-ligand complexes. The best docking binding-interaction from each laccase-ligand conformation complexes suggest great ability of these ligands to interact with the laccase active-binding site. Herein, FEB values (kcal/mol) were obtained with higher affinity values for reference controls like 2,6 - dimethoxyphenol with -4.8 Kcal/mol and ABTS with -7.1 Kcal/mol. Furthermore, the FEB values were -4.7, -6.5, -6.8, -5.2 and -6.5 Kcal/mol, for 2,4 - dichlorophenol, benzidine, sulfisoxazole, tetracycline and trimethoprim respectively with high prevalence of hydrophobic interaction with functional laccase binding residues. Lastly, this study presents for first time at the bioinformatics field a molecular docking approach for the prediction of potential substrate of laccase from Ganoderma weberianum towards biotechnological application.
G Barrera Torres*, JC Rodriguez Urbina, Renivaldo Jose dos Santos, Flavio C Cabrera, Juan C Sánchez G and Jaime A Jaramillo Carvalho
Published on: 6th December, 2023
In this review, latex from the Hevea Brasiliensis tree is presented as a material originating from nature, used to produce natural rubber, raw material for countless products for industry, as well as the manufacture of preservatives, and gloves, among others. Nowadays, its potential is expanding into various technological areas, one of which is biomedical, as described in this article. Latex has the possibility of being preserved using ammonium, which is a chemical of high health risk, but nowadays it is being substituted by acid systems excluding traditional chemicals. With these new preservation processes, the prospective use of latex for the design of biosensor scaffolds for drug release, and as membranes that can replace synthetic latex in patches for bioremediation in soft and bone tissues, synthetic muscles, membranes for biological micro laboratories, among others, is being studied to optimize its possibilities.
Leila Thaise Santana de Oliveira Santos*, Kayque Frota Sampaio, Elisa Esposito, Elinalva Maciel Paulo, Aristóteles Góes-Neto, Amanda da Silva Souza and Taise Bomfim de Jesus
Published on: 9th February, 2024
The city of Santo Amaro (Bahia, Brazil) gained visibility among the scientific community due to the contamination of the Subaé River by lead and cadmium from the PLUMBUM Mineração e Metalurgia Ltda industry, on the banks of the river in 1956, which produced lead ingots The present work aimed to investigate the adsorption capacity of heavy metals (Pb and Cd) of EPS produced by bacterial species from the Subaé River, for possible future application of these biopolymers in bioremediation processes in areas impacted by the aforementioned heavy metals. Subaé river water was collected for physical-chemical analysis and bacterial isolation. It was verified that all isolated bacteria produced an expressive amount of Exopolysaccharide (EPS). Thus, the optimization of this production in different sugars (sucrose, glucose, and mannitol) and in three different pHs: 5.5; 6.5, and 7.5. All bacteria produced EPS in large quantities and the best sugar was sucrose at pH 7.5. In order to use the EPS for the bioremediation area, the adsorption test of lead and cadmium was carried out by the isolated EPS. 0.5 g of the EPS was dissolved in 50 ml of deionized water, then the solutions of metals, lead acetate, and cadmium sulfate (procedure performed separately) were incubated at 28 °C for 16 h after that period, and were centrifuged. Samples were filtered to separate the insoluble EPS and the filtrates obtained were used in the quantification of the metals by atomic absorption (FAAS- Flame Atomic Absorption Spectrometry). Bacillus spp., Bacillus cereus, Staphylococcus spp., and Serratiamarcescens, all showed tolerance to the tested metals, due to the efficiency in the adsorption capacity of the EPS, and it was possible to distinguish seven genera, Klebsiella pneumonia, Pseudomonas aeruginosa, Lysinibacillus spp. to be used in the bioremediation of environments contaminated with heavy metals.
John Sunday Uzochukwu, Nweke Chinenyenwa Nkeiruka*, Nwachukwu Josiah Odinaka and Olufemi Gideon Olajide
Published on: 27th January, 2025
Alginate, a naturally occurring biopolymer extracted from brown algae, presents a promising avenue for developing sustainable and efficient membranes for wastewater treatment. This review comprehensively examines recent advancements in the fabrication, modification, and application of alginate-based membranes for effective water purification. The paper delves into various fabrication techniques, including casting, electrospinning, and 3D printing, which influence the structural and functional properties of the resulting alginate membranes. To enhance performance, strategies such as crosslinking, incorporation of porogens, and surface functionalization are employed. These modifications optimize crucial properties like mechanical strength, porosity, selectivity, and antifouling resistance. Furthermore, Response Surface Methodology (RSM) has emerged as a valuable tool for systematically optimizing fabrication parameters, enabling researchers to identify optimal conditions for achieving desired membrane characteristics. The integration of alginate membranes with biological treatment processes, such as phycoremediation (utilizing microalgae) and mycoremediation (employing fungi), offers a synergistic approach to enhance wastewater treatment efficiency. By immobilizing these microorganisms within the alginate matrix, their bioremediation capabilities are amplified, leading to improved pollutant degradation and nutrient removal. In conclusion, alginate-based membranes demonstrate significant potential as a sustainable and effective technology for wastewater treatment. Continued research and development, focusing on optimizing fabrication processes and exploring innovative integration strategies with biological systems, will further advance the application of alginate membranes in addressing the pressing global challenge of water pollution.
Many thanks for publishing my article in your great journal and the friendly and hassle-free publication process, the constructive peer-review, the regular feedback system, and the Quick response to a...
Azab Elsayed Azab
I, Muhammad Sarwar Khan, am serving as Editor on Archives of Biotechnology and Biomedicine (ABB). I submitted an editorial titled, 'Edible vaccines to combat Infectious Bursal Disease of poultry' for ...
University of Agriculture, Pakistan
Muhammad Sarwar Khan
Service and process were excellent as was the “look” of the article when published.
Deane Waldman
“The choice to submit the forensic case study to the Journal of Addiction Therapy and Research was dictated by the match between the content and the potential readership. The publication process pro...
Elisabeth H Wiig
Your services are very good
Chukwuka Ireju Onyinye
To the editorial team at HSPI and the Journal of Clinical Nephrology:
Thank you so much for your hard work and collaboration in bringing our article to life. Your staff was responsive, flexible, and ...
Alejandro Munoz
Journal of Pulmonary and Respiratory Research is good journal for respiratory research purposes. It takes 2-3 weeks maximum for review of the manuscript to get published and any corrections to be made...
Divya Khanduja
The service from the journal staff has been excellent.
Andy Smith
In 2017, I submitted a manuscript to the journal Archives of Biotechnology and Biomedicine belonging to Heighten Science Publications Corporation. Within one week I already received the response from ...
University of Tubingen, Germany
Yvonne Mast
I hope to ability to make some new investigation and publish in Your Company in future.
HSPI: We're glad you're here. Please click "create a new Query" if you are a new visitor to our website and need further information from us.
If you are already a member of our network and need to keep track of any developments regarding a question you have already submitted, click "take me to my Query."