Validation of Kinetic Stem Cell (KSC) counting algorithms for rapid quantification of human hematopoietic stem cells
Published on: 28th November, 2022
Specific quantification of therapeutic tissue stem cells (TSCs) is a major challenge. We recently described a computational simulation method for accurate and specific counting of TSCs. The method quantifies TSCs based on their unique asymmetric cell kinetics, which is rate-limiting for TSCs’ production of transiently-amplifying lineage-committed cells and terminally arrested cells during serial cell culture. Because of this basis, the new method is called kinetic stem cell (KSC) counting. Here, we report further validations of the specificity and clinical utility of KSC counting. First, we demonstrate its quantification of the expected increase in the hematopoietic stem cell (HSC) fraction of CD34+-selected preparations of human-mobilized peripheral blood cells, an approved treatment product routinely used for HSC transplantation therapies. Previously, we also used the KSC counting technology to define new mathematical algorithms with the potential for rapid determination of TSC-specific fractions without the need for serial culture. A second important HSC transplantation treatment, CD34+-selected umbilical cord blood (UCB) cells, was used to investigate this prediction. We show that, with an input of only simple population doubling time (PDT) data, the KSC counting-derived “Rabbit algorithms” can be used to rapidly determine the specific HSC fraction of CD34+-selected UCB cell preparations with a high degree of statistical confidence. The algorithms define the stem cell fraction half-life (SCFHL), a new parameter that projects stem cell numbers during expansion culture. These findings further validate KSC counting’s potential to meet the long-standing unmet need for a method to determine stem cell-specific dosage in stem cell medicine.
Evaluation of Performance Research Nuclear Reactors' Steady-state and Kinetic Model Analyses
Published on: 18th June, 2024
The mainstays of nuclear substance radiation and isotopic synthesis are nuclear-powered power plants, however effective safety evaluation is made tougher by the complicated construction topologies and physical connection effects. This work proposes a multiphysics-linked technique for evaluating both the kinetic and steady-state behaviors of the MPRR and LVR-15 laboratory reactors. To represent complicated member geometries, homogenized assembling sections are generated using two-dimensional whole-core computational simulations. It is discovered that the steady-state findings and the so-called Monte Carl solution comparisons correspond quite nicely. The greatest assemble power mistakes for LVR-15 and MPRR are 6.49%/10%, and the highest command rod value mistakes are 31 pcm/136 pcm, and the mistakes are 377 pcm/383 pcm, accordingly. Meanwhile, the study is done on transitory procedures, such as reactivity-initiated disasters and exposed loss-of-flow mishaps. Both units' modeling findings show plausible adverse feedback events. Furthermore, it is shown that the two reactors' accident-related behaviors are comparable though having different core architectures since they employ the exact same kinds of water as a fluid. The technique for studying nuclear power plant kinetics known as Multi-Physics Simulation (MPM) is explained. Drawing on many research and verification efforts conducted at Politecnico di Milan, Italy, MPM is shown to be a valuable instrument for managing reactors' security and oversight. It may be viewed as a holistic analytical tool that is implemented during the reactor architecture design phase. The capacity to concurrently answer the interrelated equations that control the many physical processes taking place in a nuclear plant inside the same simulated setting is a core characteristic of MPM.
Computational Simulation of Phase-Molecular Separation-DNA/RNA-Related Function Based on Gene Ontology using Combination of Computational Fluid Dynamics, Machine Learning and Membrane Systems
Published on: 21st May, 2025
Our evaluation and its outcomes/outcomes/hints spotlight that gaining a (having to do with measuring matters with numbers) knowledge of the proteome company in living cells, and its outcomes/consequences/tips for the (introduction and production/ organization of objects) of condensates and MLOs, is a critical assignment that the section separation field wishes to face/address. Our findings that dosage-sensitive (tiny chemical meeting commands interior of living things), insufficient (tiny chemical meeting commands internal of living things) and homologs especially, are overrepresented amongst human LLPS drivers, spotlight furthermore the needed component of preserving the mobile (oversupply/huge quantity) of the (bearing on everyone or issue) DNA/RNA merchandise at a great degree well suited with tightly managed LLPS conduct, to keep away from extreme (diseases/the have a look at of diseases) that unexpected errors in any direction may also cause. In-depth close interest of the records on DNA/RNA concentrations used in the LLPS experiments assisting our excessive self-belief dataset of human driver DNA/RNA s laid the uncertainties related with defining the frame-shape-related meaningful ranges of this essential restriction/guiding principle that leads and controls condensate (introduction and production/ organization of items), and recommended how those uncertainties can be lessened (something awful) and (ultimately) shortened.Graphical abstract: Computational Simulation of Phase-Molecular Separation-DNA/RNA-Related Function Based on Gene Ontology Using Combination of Computational Fluid Dynamics, Machine Learning and Membrane Systems.
Search by University/Institution
Enter your University/Institution to find colleagues at HSPI.
Ensuring author's satisfaction with
- Friendly and hassle-free publication process
- Less production time of articles
- Constructive peer-review
- Enhancing journal reputation
- Regular feedback system
- Quick response to authors' queries
Most Viewed Keywords
- Atrial fibrillation
- WPW pattern
- Pharmaco-economic study
- Pollinosis
- Fecal microbiota transplantation treatment
- Intracerebral hemorrhage
- Well-being
- Gyroscope theory
- One-work-free-afternoon
- Progressive myopia
- Inoculation
- Stomach cancer
- Lower urinary tract disease
- Hydrogel
- Body fluids identification
- Joint Vibration Analysis (JVA)
- Hypertension
- Cystic adventitial disease
- Useful field concepts
- Down syndrome
Search Articles by Country
Get all latest articles in all Heighten Science Publications Inc journals by country.
Testmonials
The Journal Clinical Nephrology provides a good opportunity for readers to stay updated in the field of clinical nephrology. Additionally - it provides a good opportunity for authors to publish their...
Assaf Harofeh Medical Center, Israel
Leonid Feldman
The services of the journal were excellent. The most important thing for an author is the speed of the peer review which was really fast here. They returned in a few days and immediately replied all o...
Eastern Mediterranean University, Cyprus
Zehra Guchan TOPCU
Dear colleagues! I am satisfied with our cooperation with you. Your service is at a high level. I hope for a future relationship. Let me know if I can get a paper version of the magazine with my artic...
Aksenov V.V
Thank you very much for accepting our manuscript in your journal “International Journal of Clinical Virology”. We are very thankful to the esteemed team for timely response and quick review proces...
Abdul Baset
I, Muhammad Sarwar Khan, am serving as Editor on Archives of Biotechnology and Biomedicine (ABB). I submitted an editorial titled, 'Edible vaccines to combat Infectious Bursal Disease of poultry' for ...
University of Agriculture, Pakistan
Muhammad Sarwar Khan
I very much appreciate the humanitarian services provided in my stead by this journal/publisher. It exhibits total absence of editorial impertinence. As an Author, I have been guided to have a fruitf...
Chrysanthus Chukwuma
"It was a pleasure to work with the editorial team of the journal on the submission of the manuscript. The team was professional, fast, and to the point".
NC A&T State University, USA
Moran Sciamama-Saghiv
I am glad to submit the article to Heighten Science Publications as it has a very smooth and fast peer-review process, which enables the researchers to communicate their work on time.
Anupam M
“It was a delightful experience publishing my manuscript with the Clinical Journal of Obstetrics and Gynecology. They offered me lots of opportunities I never had from most publishing houses and the...
Department of Agricultural Economics, Agribusiness...
Akowuah Jones Asafo
My candid opinion is that the service you render is second to none. My favourite part is the prompt response to issue, really i value that.