Validation of Kinetic Stem Cell (KSC) counting algorithms for rapid quantification of human hematopoietic stem cells
Published on: 28th November, 2022
Specific quantification of therapeutic tissue stem cells (TSCs) is a major challenge. We recently described a computational simulation method for accurate and specific counting of TSCs. The method quantifies TSCs based on their unique asymmetric cell kinetics, which is rate-limiting for TSCs’ production of transiently-amplifying lineage-committed cells and terminally arrested cells during serial cell culture. Because of this basis, the new method is called kinetic stem cell (KSC) counting. Here, we report further validations of the specificity and clinical utility of KSC counting. First, we demonstrate its quantification of the expected increase in the hematopoietic stem cell (HSC) fraction of CD34+-selected preparations of human-mobilized peripheral blood cells, an approved treatment product routinely used for HSC transplantation therapies. Previously, we also used the KSC counting technology to define new mathematical algorithms with the potential for rapid determination of TSC-specific fractions without the need for serial culture. A second important HSC transplantation treatment, CD34+-selected umbilical cord blood (UCB) cells, was used to investigate this prediction. We show that, with an input of only simple population doubling time (PDT) data, the KSC counting-derived “Rabbit algorithms” can be used to rapidly determine the specific HSC fraction of CD34+-selected UCB cell preparations with a high degree of statistical confidence. The algorithms define the stem cell fraction half-life (SCFHL), a new parameter that projects stem cell numbers during expansion culture. These findings further validate KSC counting’s potential to meet the long-standing unmet need for a method to determine stem cell-specific dosage in stem cell medicine.
Pulmonary edema ex vacuo or unilateral shock lung: a case report
Published on: 25th November, 2022
Pulmonary edema is a rare but potentially life-threatening iatrogenic complication after treatment through therapeutic thoracentesis of a collapsed lung due to a hydro- or pneumo-thorax.
Integrated vermicomposting and green roof techniques for food production in urban and rural areas
Published on: 5th December, 2022
Thousands of tons of biodegradable organic waste generates in urban and rural areas every day, creating disposal problems. Urban organic waste can be converted into valuable output products (vermicompost, vermin-liquid, and earthworms) by applying a vermicomposting technique that had different. Implementing green roofs via soilless culture systems as micro-scale farms led to increasing natural resource use efficiencies as well as producing fresh food. The integration of both techniques will create not just reduce pollution and climate change impacts but also for increasing food production and security in urban, enhance the lifestyle and increase public awareness of environmental issues. This process is profitable at any scale of operation.
The smart agriculture applications (current and future)
Published on: 5th December, 2022
Smart agriculture applications (monitoring, sensing, automation and control) of micro-climate and environmental conditions for different agriculture production sectors and scales, decision-makers and researchers need to take it into consideration to strengthen the efforts of mitigation and adaption of climate change impacts as well as maximize the natural resources use efficiencies and food production. Motivate the farmers to implement smart agriculture applications, especially in developed and poor countries, strong cooperation for technology transfer and build up the technology infrastructure of information and communication (ICT) plus the internet of things (IoT).
Oncocardiology: Far beyond the cardiotoxicity
Published on: 29th November, 2022
Approximately 73.4% of global deaths are caused by chronic non-communicable diseases, among them, cardiovascular and cerebrovascular diseases, tumors, and chronic respiratory diseases ranked in the top 3 respectively [1]. An accumulating body of evidence showed that the risk of all-cause mortality in cancer patients with cardiovascular disease (CVD) was 3.78 times higher than that of those without CVD and 8.8% of cancer survivors died of CVD [2]. Heart failure (HF) is a serious manifestation or terminal stage of various heart diseases. Although myocardial damage and dysfunction are the main causes of HF, the cardiovascular injury caused by the tumor itself and the detrimental effect of cancer treatment also play an important role. More recently, the data has suggested that up to 25% - 30% of patients with HF have histories of cancer for about 10 years; and cancer also determines the prognosis of heart HF [3].
The application of therapeutic exercise as a means of rehabilitation in a patient diagnosed with MS, Case Study
Published on: 5th December, 2022
Multiple Sclerosis (MS) is a chronic and often progressive condition that affects the Central Nervous System (CNS) and as a result has negative consequences on a person’s physical condition, functioning, mood, and Activities of Daily Living (ADL) [1].
Pain and culture - on cultural communications of suffering
Published on: 29th November, 2022
The impact of culture on the communication of pain becomes visible in a comparative perspective when individuals with two different cultures meet.Regardless of the physiological cause, it is through lived experience, in social life, that it is decided how, where, when and for whom pain is to be communicated. Age and gender, situation and context as well as the type of pain are essential ingredients in communication. The communication is always adapted to expectations from those present, their own experiences of pain and the specific circumstances, the cause of the pain. The impact of culture on the communication of pain becomes visible in a comparative perspective when individuals with two different cultures meet.
Ecology of vital activity as an element of antistress therapy on the example of the organization of the work of a medical center with industrial enterprises under the VMI program
Published on: 29th November, 2022
The pandemic the new Coronavirus infection has brought changes in the health status of a large number of our citizens. The consequence of this has been a higher rate of employees seeking medical care and subsequent sick leave. Crisis phenomena in the economic and social life of society are associated with the consequences of the pandemic, moreover, the stress burden on the population increases with the consequences of the pandemic. All this affects the level of performance. The transferred COVID-19 has increased the number of patients with complaints of pain in joints and muscles, with the phenomena of cardiovascular pathology, as well as in breathing, increased frequency of asthma attacks, etc. That is, complications affected a wide range of diseases [1-4]. All this is the consequence, including neurological pathology, which is closely related to stress. According to surveys of several large industrial enterprises, 31.7% of those seeking medical help report anxiety, sleep problems, and a feeling of fatigue [5]. Exacerbation of chronic diseases was recorded in more than 50% of patients with COVID-19. The figures show that more than 1/3 of the population is chronically unwell, and more often than usual they apply for sick leave, which inevitably leads to a decrease in economic indicators [6-10].
Research progress on marker-assisted selection pyramiding breeding of disease resistance genes in wheat
Published on: 30th November, 2022
Marker-Assisted Selection (MAS) pyramiding breeding combined with traditional breeding techniques has become an important method for molecular breeding in wheat. In recent years, with the continuous discovery of disease-resistance genes and the development of molecular markers associated with related genes in wheat, the research of MAS pyramiding disease-resistance genes has made great progress. The progress on the study of MAS pyramiding resistance genes in wheat powdery mildew, rust, and Fusarium head blight (Fhb) was reviewed. The aims of pyramiding breeding were discussed in order to promote the research on MAS pyramiding breeding in wheat.
A look at polyploidy and plant breeding
Published on: 28th November, 2022
Polyploidization is a process that generates genetic variability and therefore one of the engines of biological evolution. Since polyploidization produces important changes in the phenotype, mainly an increase in the size of the organs (i.e.: flowers and fruits), it is also a very important and powerful tool for plant improvement. Despite its intense use in breeding programs for various species, very little is known so far about the nature of this phenomenon. This work presents a brief review of the results obtained by the use of this tool in plant breeding and also raises some reflections on its mechanism of action.
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