Solid Garbage Treatment: Issues and Challenges
Published on: 16th July, 2025
The escalating volume of solid waste due to rapid urbanization, population growth, and industrialization poses significant environmental and public health challenges. Improper disposal of solid waste, especially organic and municipal garbage, leads to pollution of soil, air, and water, causing a surge in health risks and ecosystem degradation. Traditional waste management practices are often inefficient, expensive, and time-consuming. Composting and vermicomposting have emerged as eco-friendly, cost-effective, and sustainable alternatives for organic waste management. Vermicomposting, which employs earthworms to decompose organic waste into nutrient-rich compost, has shown potential in improving soil fertility, reducing pathogenic microbes, and recycling essential nutrients like nitrogen, phosphorus, and potassium. Various organic wastes such as agricultural residues, municipal waste, animal dung, and market garbage have been effectively treated through vermicomposting using species like Eisenia fetida and Eudrilus eugeniae. This review emphasizes the technological, environmental, and agricultural benefits of vermicomposting in transforming biodegradable solid waste into valuable compost, promoting sustainable waste management practices, and enhancing agricultural productivity.
Wild-type Agrobacterium rhizogenes-mediated gene transfer in plants: Agrobacterium virulence and selection of transformants
Published on: 12th June, 2017
OCLC Number/Unique Identifier: 7286428023
Agrobacterium rhizogenes ATCC 15834 wild type strain was transformed with the binary vector pBI121 using the heat shock method. The transformed Agrobacterium was then tested for virulence through tobacco leaf explant transformation. Compared to the non-transformed Agrobacterium, the transformed Agrobacterium showed reduced virulence, producing significantly lower number of hairy roots in tobacco leaf explants. Although the transformed Agrobacterium showed reduced virulence, it was able to transfer the T-DNA of the binary vector into the plant genome, resulting in stable GUS expression in the generated hairy roots. This indicated that in addition to the transfer DNA (T-DNA) from its root inducing (Ri) plasmid, the transformed Agrobacterium is also capable of transferring the binary vector T-DNA and allowing the integration of a foreign gene. Results also showed that hairy root generation efficiency of the transformed Agrobacterium varied with the concentration of the selection agent (kanamycin). Hairy root generation efficiency (hairy roots·explant-1) progressively increased with decreasing concentrations of kanamycin; and the efficiency was highest in the absence of kanamycin. Generated hairy roots showed very strong to tiny GUS expression even those that grew under the highest concentration of the kanamycin (50 mg·L-1). This indicated that co-transformation and efficient transgene expression does not always occur.
Reaction of Psidium guineense and Psidium guajava genotypes to infection of Meloidogyne enterolobii
Published on: 8th February, 2018
OCLC Number/Unique Identifier: 7347029155
Psidium guajava (guava) is an important crop and economic resource in many tropical countries and Brazil stands out as one of its major commercial producers. The guava crop has been severely attacked by the plant parasitic nematode Meloidogyne enterolobii which has caused drastic reduction of productivity and, in some cases, even total loss of cultivated areas. The use of resistant rootstocks that are tolerant to these soil pathogens can be a low-cost solution to this pathogen, which has established itself as one of the major constraints for the cultivation of guava in Northeast Brazil. The objective of this work was to evaluate the reaction of P. guajava and P. guineense to M. enterolobii infection under greenhouse conditions, with an infection period of 60 days. 12 P. guineense, and 4 P. guajava were evaluated. The host response to the nematode infection was evaluated according to the following parameters: gill index (GI), reproductive factor (RF), and reduction of reproductive factor (RRF). Considering the RRF criteria, the P. guineense genotypes, AR1, AR3, AR4 and AR10 were considered resistant. The RF index was considered inadequate to evaluate resistance in the 60 days period, due the slow development of the parasite in P.guineense.
Role of HECT ubiquitin protein ligases in Arabidopsis thaliana
Published on: 20th March, 2018
OCLC Number/Unique Identifier: 7794148150
Ubiquitination is a kind of posttranslational modification of proteins in eukaryotes, and it plays an important role in the growth and development of organisms. The ubiquitination of proteins is a cascade enzymatic reaction involving three enzymes. The homologous to E6-AP carboxy terminus ubiquitin-protein ligases (HECT E3s) family is an important ubiquitin-protein ligases family. The family all have a HECT domain of approximately 350 amino acids in the C-terminus. However, studies on plant HECT E3s, such as structural features, prediction of HECT domain function, and their regulatory mechanisms, are very limited. In this paper, Arabidopsis thaliana HECT family genes were analyzed, including gene structure and functional domains and its limited known functions in protein degradation, gene transcription regulation, epigenetically regulation or other functions, finally speculate their roles in plant morphologies, aging or responsive to environmental stress.
Detection of Clavibacter michiganensis subsp. michiganensis in tomato and chili seeds and farming area of Sinaloa, Mexico
Published on: 8th June, 2018
OCLC Number/Unique Identifier: 7814980304
Phytosanitary inspectors play an important role in diagnosing diseases in foreign plant material. However, some deficiencies have been detected in the detectionc ausing the entrance of many microorganisms. Therefore, it was of great interest to detect the presence of Clavibacter michiganensis subsp. michiganensis (Cmm) in foreign tomato and chili seed in the agricultural area of Sinaloa, Mexico, besides the growth and cell density of Cmm was evaluated in different selective media under continuous illumination and photoperiod. The results indicate that seeed of 35 varieties of tomatoes was collected; while for Chili seed were 18. This study was supported by farmers (225) which represent 79% of all growers and 32 business engaged in the sale of agro-supplies, provided seeds of varieties and hybrids. Those growers are from six areas (Culiacan, El Tamarindo, Navolato, Culiacan, El dorado and Badiraguato). For detection of Cmm in tomato seed, from 35, only four was variability considering Immunochromatography and ELISA techniques; however, considering chemical and physiological test, the result was negative. Similar results were in 18 varietes of chili seed, where eight showed variability to detect Cmm, and negative by chemical and physiological test. According to the growth and cell density of Cmm, the optimal medium was YDC under pH stable and continuous light conditions. It is recommended to consider the fusion of diagnostic techniques in the emission of a result.
Bacillus amyloliquefaciens as a plant growth promoting bacteria with the interaction with of grass salt Distichlis palmeri (Vasey) under field conditions, in desert of Sonora, Mexico
Published on: 2nd October, 2018
OCLC Number/Unique Identifier: 7900050235
The halophyte Distichlis palmeri (Vasey) is a plant resource with high potential to be harvested in the coastal areas of northwestern Mexico; enlarge the knowledge and domestication for its incursion into the agricultural sector, plays an important role for arid areas with saline intrusion problems. However, its productivity depends on the supplementary supply of nitrogen, as well as other essential macro and micronutrients. The microorganisms considered beneficial are an alternative to chemical fertilization, highlighting those Plant Growth Promoting Bacteria (PGPB). In the present study, the inoculation of the Bacillus amyloliquefaciens (B.a.) as a halobacterium PGPB was evaluated to know the response in seeds of Distichlis spicatai obtained from natural population from colorado river in Delta north of the Gulf of California. Wild seed was collected and germinated previously inoculated with B. a., and sowed in germinated beds. Later, seedlings were planted under field and salinity conditions in the coast of Hermosillo, Sonora. Three treatments were examined (T1: B.a., T2: Chemical fertilization, T3: Negative control), with four repetitions each treatment. Each repetition consisted of experimental plots of 5 x 5 m, with a separation of 1 m between them. The harvest was carried out 600 days after sowing. The results indicate that treatments inoculated with halobacteria B.a., showed significant results in crude protein, non-protein nitrogen, neutral detergent fiber and acid detergent fiber, as well as spike length and number of seeds. The results obtained suggests the feasibility of biofertilizers where biomass and seed production are significant compared to non-inoculated controls.
Primer Pairs for Rice (Oryza sativa L.) Bisulfite Sequencing Studies
Published on: 6th November, 2018
OCLC Number/Unique Identifier: 7905997684
Many agriculturally important properties such as heterosis, inbreeding depression, phenotypic plasticity, and resistance for biotic and abiotic stresses are thought to be affected with epigenetic components. New discoveries related with epigenetics are likely to have a major impact on strategies for crop improvement in rice breeding. However, assessing the contribution of epigenetics to heritable variation in plant species still poses major challenges. Methylation of cytosine in DNA is one of the most important epigenetic mechanisms in plants. DNA methylation not only plays significant roles in the regulation of gene activity, but also it is related with genomic integrity. Although most of next generation sequencing (NGS) technologies do not require the use of target specific primer pairs to identify and study DNA cytosine methylation, validation studies of NGS uses selective primer pairs. Bisulfite sequencing technique is a gold method for DNA cytosine methylation studies. However, bisulfite sequencing requires the development of bisulfite primer pairs to selectively study DNA sequences of interest. In this study 9 bisulfite specific primer pairs were identified and validated. These primer pairs successfully amplified bisulfite converted and unconverted genomic DNA extracted from radicle and plumule of rice (Oryza sativa L.) seedlings. Results of the present study clearly revealed the occurrence of CG, CHG and CHH (H stands for C. T or A nucleotides) contents in studied DNA sequence targets were different indicating potential role of DNA cytosine methylation in these genes. Primer pairs reported in this study could be used to detect DNA methylation which is one of the most important epigenetic mechanisms affecting the development, differentiation or the response to biotic and abiotic stress in rice (Oryza sativa L.).
Seasonal Influenza in Morocco
Published on: 18th September, 2025
Influenza is an acute respiratory infection caused by influenza viruses of the Orthomyxoviridae family. Influenza virus cell culture consists of inoculating viral isolates on an MDCK cell layer. The objective of this work is to describe the role of cell culture in influenza virus subtyping during the 2023/2024 influenza epidemic at the Mohammed V Military Teaching Hospital - Rabat.This is a prospective descriptive study carried out at the Virology laboratory of the HMIMV in Rabat, during the 2023/2024 influenza epidemic season (between October and May). It included all samples from patients positive for influenza viruses by the respiratory PCR test. Of the 86 samples positive by PCR, 36 were selected for viral culture.Cell culture allowed the isolation of the virus from the original sample, thus producing a sufficient quantity for further antigenic and genetic characterization, allowing the subtyping of circulating strains.The results showed a predominance of influenza A(H3N2) with 35 cases out of 36 compared to influenza A(H1N1) pdm09 with only 1 case.Cell culture also makes it possible to assess the sensitivity of isolated viral strains to antiviral molecules and to detect the emergence of resistance. Thus, it plays an important role in virological surveillance and in alerting to the event of the appearance of new viruses.
Prevalence of West Nile Virus among Sudanese Participants in Gezira State
Published on: 22nd September, 2025
West Nile virus (WNV), a mosquito-borne pathogen belonging to the Flavivirus genus (family Flaviviridae), is primarily responsible for West Nile fever. In rare cases (< 1% of infections), it can lead to West Nile neuroinvasive disease (WNND), which carries an approximate fatality rate of 10%. This cross-sectional study aimed to assess WNV exposure among residents of Gezira State, Sudan, from 2019 to 2022. A total of 400 participants were included, with 5 mL of blood collected from each—2 mL in an EDTA tube and 3 mL in a plain container. Plasma and serum were separated via centrifugation, and WNV-specific IgG and IgM antibodies were detected using ELISA. The results revealed that 62.25% (249 cases) tested positive for WNV-IgG antibodies, while 37.75% (151 cases) were negative. None of the samples showed detectable WNV-IgM antibodies. Statistical analysis indicated no significant association between WNV-IgG seropositivity and age or gender (p = 0.060 and p = 0.117, respectively). However, significant correlations were observed with marital status and education level (p < 0.05). The study highlights the need for ongoing surveillance of WNV and other vector-borne diseases in the region to identify active West Nile Virus infections. Public health efforts should prioritize mosquito control measures, community education, and awareness campaigns about WNV symptoms and prevention.
Estimation of Radiation Dose to Blood Vessels and Components from Medical Imaging Procedures: Current Status
Published on: 8th May, 2025
Aim: This study aims to determine the current status for estimation of radiation dose to blood vessels and components from medical imaging procedures.Methodology: A database search on internet via PubMed and Google Scholar was performed to find published papers in estimation of radiation dose to blood vessels and components from medical imaging procedures. Results: Few published papers were found; namely two published papers. Radiation dose to blood vessels and components were assumed to be included in total radiation dose estimation for organ or tissue, without considering different in radiosensitivity. Conclusion: It seems that effect of radiation on blood vessels and components is underestimated, in ICRP 60 and 103 recommendations reports. Recommendation: It is recommended to conduct more studies to estimate radiation dose for blood vessels and components from medical imaging procedures and revise the value of tissue weighting factor for bone marrow.
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