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Influenza is an acute respiratory infection caused by influenza viruses of the Orthomyxoviridae family. Influenza virus cell culture consists of inoculating viral isolates on an MDCK cell layer. The objective of this work is to describe the role of cell culture in influenza virus subtyping during the 2023/2024 influenza epidemic at the Mohammed V Military Teaching Hospital - Rabat.This is a prospective descriptive study carried out at the Virology laboratory of the HMIMV in Rabat, during the 2023/2024 influenza epidemic season (between October and May). It included all samples from patients positive for influenza viruses by the respiratory PCR test. Of the 86 samples positive by PCR, 36 were selected for viral culture.Cell culture allowed the isolation of the virus from the original sample, thus producing a sufficient quantity for further antigenic and genetic characterization, allowing the subtyping of circulating strains.The results showed a predominance of influenza A(H3N2) with 35 cases out of 36 compared to influenza A(H1N1) pdm09 with only 1 case.Cell culture also makes it possible to assess the sensitivity of isolated viral strains to antiviral molecules and to detect the emergence of resistance. Thus, it plays an important role in virological surveillance and in alerting to the event of the appearance of new viruses.

West Nile virus (WNV), a mosquito-borne pathogen belonging to the Flavivirus genus (family Flaviviridae), is primarily responsible for West Nile fever. In rare cases (< 1% of infections), it can lead to West Nile neuroinvasive disease (WNND), which carries an approximate fatality rate of 10%. This cross-sectional study aimed to assess WNV exposure among residents of Gezira State, Sudan, from 2019 to 2022. A total of 400 participants were included, with 5 mL of blood collected from each—2 mL in an EDTA tube and 3 mL in a plain container. Plasma and serum were separated via centrifugation, and WNV-specific IgG and IgM antibodies were detected using ELISA. The results revealed that 62.25% (249 cases) tested positive for WNV-IgG antibodies, while 37.75% (151 cases) were negative. None of the samples showed detectable WNV-IgM antibodies. Statistical analysis indicated no significant association between WNV-IgG seropositivity and age or gender (p = 0.060 and p = 0.117, respectively). However, significant correlations were observed with marital status and education level (p < 0.05). The study highlights the need for ongoing surveillance of WNV and other vector-borne diseases in the region to identify active West Nile Virus infections. Public health efforts should prioritize mosquito control measures, community education, and awareness campaigns about WNV symptoms and prevention.

Medical postgraduate trainees are given increasing levels of responsibility during training in the apprenticeship-model of training [1-3]. Responsibility is said to be a key driver of deep learning and understanding [4-7]. Trainees with greater levels of responsibility for decision making have higher levels of motivation to learn compared with trainees who self-assess as having less autonomy [5]. The Royal College of Pathologists (RCPath) indicate that ‘graded responsibility’ is part of training histopathologists and provided a framework for implementation with increasingly complex specimens suitable for reporting by more senior trainees [8,9]. 

The limits of classical equivalent computation based on time, dose, and fractionation (TDF) and linear quadratic models have been known for a long time. Medical physicists and physicians are required to provide fast and reliable interpretations regarding the delivered doses or any future prescriptions relating to treatment changes. In this letter, we propose an outline related to the different models usable for equivalent and biological doses that are likely to be the most appropriate. The used methodology is based on: the linear-quadratic-linear model of Astrahan, the repopulation effects of Dale, and the prediction of multi-fractionated treatments of Thames.

Purpose: Stroke frequently causes severe deficits in upper limb function, which makes it difficult for patients to carry out daily tasks. An organized home-based intervention called the Graded Repetitive Arm Supplementary Program (GRASP) was developed to help stroke patients regain function in their hands and arms. This systematic review assesses the data demonstrating beneficial effects of the GRASP approach on hand function, gross and fine motor control of the upper limb in individuals post-stroke.Methods: A systematic search was performed in databases including PubMed, Scopus, CINAHL, and Cochrane Library up to April 2025. We searched the data using Keywords such as “GRASP,” “Graded Repetitive Arm Supplementary Program,” “stroke rehabilitation,” “upper limb recovery,” “fine motor skills,” and “gross motor control.” Inclusion criteria were randomized controlled trials (RCTs), quasi-experimental studies, and cohort studies involving adult stroke survivors undergoing GRASP. Studies were excluded if they were non-English and focused on other forms of intervention. Quality assessment was performed using the PEDro scale and Cochrane risk-of-bias tool.Results: Out of 243 studies initially screened, 8 met the inclusion criteria. Most studies reported significant improvements in upper limb function, including enhanced hand dexterity, grip strength, and coordination following participation in GRASP programs. High adherence rates and participant satisfaction were consistently noted. Some studies indicated that benefits were maintained at follow-up, suggesting long-term efficacy. Common outcome measures included the Fugl-Meyer Assessment (FMA), Box and Block Test (BBT), and Action Research Arm Test (ARAT).Discussion: The findings suggest that GRASP is an effective adjunct to conventional stroke rehabilitation, particularly for enhancing hand function and motor control. The structured yet flexible nature of GRASP allows for scalability and adaptability to various patient needs. Limitations of the reviewed studies include small sample sizes, heterogeneity in intervention duration, and variability in outcome measures. Further research with standardized protocols and larger samples is warranted.Conclusion: The GRASP approach appears to be a promising intervention for improving functional ability of the hand, and gross and fine motor control in the upper limbs post-stroke. Incorporating GRASP into home-based rehabilitation could enhance recovery and reduce long-term disability. Continued research is essential to establish best practices for implementation and to optimize patient outcomes.

Geothermal energy, with a global installed capacity of 16.7 GW by 2023 and an annual generation of more than 97 TWh, is consolidating its position as a key pillar in the energy transition. From the pioneering Lardarello plant (1913) to The Geysers complex in California (the largest in the world with 1.5 GW of capacity), this energy source has demonstrated its potential to supply baseload electricity 24 hours a day, with a carbon footprint up to 10 times lower than fossil fuels. The article explores how the architectural design of these plants integrates geological, environmental, and technological factors. Systems such as binary cycles (which operate at 90°C and reduce emissions by 95%) allow the exploitation of low-temperature resources, expanding their applicability to non-volcanic regions. However, challenges remain: reservoir exploration is 30-40% uncertain, and initial costs exceed $4,500 per installed kW, according to the U.S. Department of Energy. Iconic cases like The Geysers illustrate innovative solutions. Since 2003, its recharge project with treated wastewater (11 million gallons/day) has revitalized the reservoir, extending its lifespan by decades. This circular economy approach not only optimizes resources but also reduces water conflicts in arid areas. The future points to disruptive designs: Enhanced Geothermal Systems (EGS), which could increase global potential tenfold by enabling dry reservoirs, and urban heating districts with heat pumps, capable of providing heating at -20°C. By 2030, drilling innovations—such as smart drill bits and robotics—could reduce costs by 50%, accelerating adoption. In a world that needs to double clean energy by 2040, geothermal energy is emerging not only as a renewable source, but as an architectural canvas where engineering and sustainability converge to redefine the energy landscape. Its evolution will depend on creatively overcoming technical barriers, transforming the Earth’s heat into the cornerstone of a decarbonized era.

Background: The motivation of this paper is to analyze the growing air pollution level in the city of Bengaluru, as it was once a green city but is now facing problems with the use of private vehicles, such as increasing levels of air pollutants. Green buildings focus on reducing their negative environmental impacts by using less energy and water, and causing less disruption to the environment during construction. Green buildings, which also strive to enhance human health through the design of healthy indoor spaces, are arguably less well-known.Objective: The main objective is to analyze the most suitable zone in Bangalore for the construction of green buildings. The review of existing green building technologies with the use of natural materials, renewable energy, water conservation, and indoor air quality.Method: The methodology will include a study of six green building literature reviews with eminent researchers and scientists. The main principles of green building include having indoor air quality of Nitrogen oxides, volatile organic compounds, and particulate matter are the most common air impurities released into the atmosphere by the variability of natural processes and human actions. The advantages of lessened energy and water use are well known, but the potential advantages of green buildings for human health have only lately come into focus. In addition, case study review for Electronic City, Whitefield, and International Airport in BangaloreResult: To address the comparative study of case study review, Bangalore city data was collected regarding the pollutant levels, then linear regression models were created and reviewed to design green buildings in the future. Conclusion: The goal of the study is to comprehend how toxins, a rise in urban population density, the usage of novel artificial resources, and traffic contamination all work together to worsen indoor air quality and increase harmful impacts on people.

As time goes on, more and more people are using drugs in dangerous ways. This rising percentage of people abusing drugs is leading to an increase in violent behaviors connected to them. The present article will summarize and present cases of mass violence caused by drugs, displaying the harm they may cause if not regulated properly. A literature search of JSTOR, AJPH, IJERPH, Journal of Addictive Reviews, Journal of Addiction Research and Therapy, Google Scholar, CDC, CNN, New York Times, Detroit News, and ABC News was conducted from February 16, 2023, to November 03, 2024. In 2016, 56.2 million people died worldwide. There are an estimated 10,000 people who are killed each year in the United States as a direct result of drugs. Worldwide, 70 million people were diagnosed with a drug use disorder. In the global general population, 0.3%-0.6% of people are diagnosed with this disorder. 10%-69% of people in prison are diagnosed as well. From 1995 to 2000, 41% of violent crimes committed against college students and 38% of violent crimes committed against non-students were committed by an offender thought to be on drugs. Drugs have a clear connection to mass violence, and further regulation is needed for the benefit of public health and safety. 

Introduction and background: Hydatid disease (HD) is a parasitic infection caused by the larval form of Echinococcus granulosus. It is endemic in regions with widespread livestock farming and close human-animal contact. Although the liver and lungs are the most frequently involved organs, rare cases of primary subcutaneous hydatid cysts have been reported, especially in the absence of visceral involvement. Gluteal localization is extremely rare and may be misdiagnosed due to its nonspecific presentation.Case presentation: We report the case of a 25-year-old woman who presented with a gradually enlarging, painless swelling over the lateral aspect of her right buttock, noted over five months. There were no systemic symptoms, and she had no history of trauma or prior medical conditions. Physical examination revealed a well-circumscribed, fluctuating, non-mobile mass measuring 5 × 4 cm with no overlying skin changes. Laboratory results were within normal limits. Ultrasound imaging revealed multiple well-defined cystic lesions in the subcutaneous tissue. Chest X-ray and abdominal ultrasound excluded hepatic or pulmonary hydatidosis. A diagnosis of primary subcutaneous hydatid cyst was made. The patient underwent pericystectomy under spinal anesthesia. Intraoperatively, typical hydatid features were noted, and the cyst cavity was thoroughly irrigated with hypertonic saline. Postoperatively, Albendazole therapy (400 mg twice daily) was administered for three months. There were no signs of recurrence during 6 months of follow-up.Discussion: Primary soft tissue hydatid cysts are rare and can mimic benign soft tissue tumors or abscesses. In endemic regions, such lesions should be carefully evaluated using imaging and clinical suspicion. The diagnosis is typically made through imaging, and definitive treatment includes surgical excision with careful handling to prevent dissemination, accompanied by pre- and postoperative anthelmintic therapy to minimize recurrence.Conclusion: This case highlights the importance of considering hydatid disease in the differential diagnosis of gluteal masses, especially in endemic areas. Prompt diagnosis and combined surgical and pharmacologic therapy can lead to excellent outcomes without recurrence.

Background: Stature is a key biological characteristic for identifying individuals, particularly in forensic investigations. While long bones are traditionally used for stature estimation due to their strong correlation with height, craniofacial structures offer an alternative in cases where only partial remains are available, given their durability and relative preservation.Objectives: This study aimed to evaluate the relationship between craniofacial anthropometric parameters and stature, and to assess their usefulness for stature estimation in the Indian population.Methods: Twelve craniofacial parameters were measured and analyzed using regression analysis, the preferred statistical method for estimating stature from skeletal dimensions. The study focused on assessing the strength of correlation between these craniofacial measurements and actual stature.Results: All twelve craniofacial parameters demonstrated a positive correlation with stature, indicating their potential utility in forensic contexts where only craniofacial remains are present. Although long bones remain the gold standard, these findings provide support for the supplementary use of craniofacial data.Conclusion: Craniofacial anthropometry can serve as a reliable method for estimating stature, especially in scenarios where long bones are unavailable. This study contributes valuable data to the limited literature on this topic in the Indian population and aligns with existing global research supporting the role of craniofacial structures in forensic identification.